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One-step preparation of S-nitrosated human serum albumin with high biological activities
http://hdl.handle.net/2298/18631
http://hdl.handle.net/2298/1863136548afa-cecd-4762-9c4a-4b56832aa5af
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NO_23_2_Tables(For NO).pdf (36.1 kB)
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NO_23_2_Fig(For NO).pdf (207.9 kB)
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NO_23_2_121-127.pdf (113.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2011-04-22 | |||||
| タイトル | ||||||
| タイトル | One-step preparation of S-nitrosated human serum albumin with high biological activities | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | human serum albumin, nitric oxide, N-acetyl-L-tryptophan, caprylic acid, S-nitrosation, ischemia-reperfusion | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ishima, Yu
× Ishima, Yu× Hiroyama, Shuichi× Ulrich, Kragh-Hansen× Maruyama, Toru× 澤, 智裕× 赤池, 孝章× Kai, Toshiya× Otagiri, Masaki |
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| 別言語の著者 |
異島, 優
× 異島, 優× 廣山, 秀一× 丸山, 徹× 澤, 智裕× 赤池, 孝章× 甲斐, 俊哉× 小田切, 優樹 |
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| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | S-Nitrosated human serum albumin (SNO-HSA) is a large molecular weight nitric oxide carrier in human plasma, and because of its many beneficial effects in different tests, it is currently under investigation as a cytoprotective agent. However, making SNO-HSA preparations is a complicated and time-consuming process. We found that binding of caprylic acid (CA) and N-acetyl-L-tryptophan (N-AcTrp) to defatted mercaptalbumin increased S-nitrosation by S-nitrosoglutathione (GS-NO) by making Cys-34 of HSA more accessible and by protecting it against oxidation, respectively. Fortunately, HSA solutions for clinical use contain high concentrations of CA and N-AcTrp as stabilizers. By making use of that fact it was possible to work-out a fast and simple procedure for producing SNO-HSA: Incubation of a commercial HSA formulation with GS-NO for only one minute results in S-nitrosation of HSA. The biological usefulness of such a preparation was tested in a rat ischemia-reperfusion liver injury model. Although our procedure for making SNO-HSA is fast and straightforward, the cytoprotective effect of the preparation was similar to, or better than, that of a preparation made in a more traditional way. The clinical development of SNO-HSA as a strong cytoprotective agent is under way using this method in collaboration with clinicians and industrial developers. | |||||
| 書誌情報 |
Nitric Oxide 巻 23, 号 2, p. 121-127, 発行年 2010-09-15 |
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| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 関連識別子 | 10.1016/j.niox.2010.05.002 | |||||
| 権利 | ||||||
| 権利情報 | © 2010 Elsevier Inc | |||||
| 情報源(ISSN) | ||||||
| 関連名称 | 10898603 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 形態 | ||||||
| 値 | 113701 bytes | |||||
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| 値 | 207901 bytes | |||||
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| 値 | 36143 bytes | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 499 | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 論文(Article) | |||||
| 資源タイプ・ローカル | ||||||
| 値 | 雑誌掲載論文 | |||||
| 資源タイプ・NII | ||||||
| 値 | Journal Article | |||||
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| 値 | text | |||||
| 資源タイプ・ローカル表示コード | ||||||
| 値 | 01 | |||||
| URL | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WNT-5017HTP-1&_user=4545557&_coverDate=09%2F15%2F2010&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_acct=C000008298&_version=1&_urlVersion=0&_userid=4545557&md5=50f652d134dd09a6f72481d07de2387d&searchtype=a | |||||
