| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-29 |
| タイトル |
|
|
タイトル |
Hyperthermia with Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
近藤, 龍也
佐々木, 一成
松山, 利奈
森野 古賀, 沙緒里
足立, 博紀
Suico, Mary A
河島, 淳司
本島, 寛之
古川, 昇
甲斐, 広文
荒木, 栄一
|
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Induction of heat shock protein (HSP) 72 improves metabolic profiles in diabetic model mice. However, its impact on pancreatic β-cells is not known. The present study investigated whether HSP72 induction can reduce β-cell stress signaling and apoptosis, and preserve β-cell mass. MIN6 cells and db/db mice were sham-treated or treated with heat shock (HS) + mild electrical stimulation (MES) to induce HSP72. Several cellular markers, metabolic parameters and β-cell mass were evaluated. HS+MES treatment or HSP72 overexpression increased the HSP72 protein levels and decreased TNF-β-induced JNK phosphorylation, ER stress and pro-apoptotic signal in MIN6 cells. In db/db mice, HS+MES treatment for 12 weeks significantly improved the insulin sensitivity and glucose homeostasis. Upon glucose challenge, a significant increase in insulin secretion was observed in vivo. Compared with sham treatment, the HSP72, insulin, PDX-1, GLUT2 and IRS-2 levels were upregulated in the pancreatic islets of HS+MES-treated mice, whereas JNK phosphorylation, nuclear translocation of FOXO1 and NF-βB p65 were reduced. Apoptotic signals, ER stress and oxidative stress markers were attenuated. Thus, HSP72 induction by HS+MES treatment protects β-cells from apoptosis by attenuating JNK activation and cell stresses. HS+MES combination therapy may preserve pancreatic β-cell volume to ameliorate glucose homeostasis in diabetes. |
| bibliographic_information |
en : Diabetes
巻 61,
号 4,
p. 838-847,
発行年 2012-04
|
| item_16_source_id_7 |
|
|
収録物識別子 |
0012-1797 |
| item_16_source_id_9 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00628057 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
493 |
| 出版者 |
|
|
出版者 |
American Diabetes Association |
| 関連 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
22362176 |
| 関連 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.2337/db11-1098 |
Figures-1.pdf (745.1 kB)
